{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"054-602-603-251-448","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"054-602-603-251-448"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10737,"type":"PATENT","title":"The University of British Columbia - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7923,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8233,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:\"univ* briti* colum*\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 7363
Search Applicants and Owners separately:\"univ* briti* colum*\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 7363
(a) retaining the cell secreting the protein within a chamber having a solid wall defining the chamber, an inlet and an outlet, wherein the volume of the chamber is from 100 pL to 100 nL;\n
(b) exposing the protein secreted by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein secreted by the cell and wherein the capture substrate is operable to bind the protein secreted by the cell to produce a bound protein;\n
(c) flowing a first fluid volume comprising the biomolecule through the inlet into the chamber over the bound protein and out the outlet, wherein the first fluid volume is in fluid communication with the capture substrate and the bound protein; and\n
(d) determining a binding kinetic measurement between the protein secreted by the cell and the biomolecule wherein the binding kinetic measurement is a Kon; a Koff; a KD, or a combination thereof and the binding kinetic measurement is based on an interaction between the bound protein and biomolecule in the chamber."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the cell is an antibody producing cell (APC), the protein secreted by the cell is an antibody and the biomolecule is an antigen."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 or claim 2, wherein the biomolecule is a fluorescently labeled antigen."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the determining binding kinetics is a measure of antigen-antibody binding kinetics."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the protein secreted by the cell is an antibody."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the antibody is a monoclonal antibody."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the biomolecule is an antigen."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the biomolecule is selected from one of the following: an antibody, a whole cell, a cell fragment, a bacterium, a virus, a viral fragment, and a protein."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the cell is maintained in the chamber with the capture substrate."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the capture substrate is a removable capture substrate."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the removable capture substrate comprises a capture substrate capable of capturing nucleic acids."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the removable capture substrate comprises a capture substrate capable of capturing both nucleic acids and antibodies."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the removable capture substrate comprises a capture substrate capable of capturing nucleic acids and a capture substrate capable of capturing antibodies."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the exposing the protein secreted by the cell to the capture substrate comprises flowing a removable capture substrate into the chamber."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the protein produced by the cell is an antigen and wherein the biomolecule is an antibody."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein the antibody is a monoclonal antibody."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein biomolecule is a fluorescently labeled antibody."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein the fluorescently labeled antibody is a monoclonal antibody."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein the determining binding interactions is a measure of antigen-antibody binding kinetics."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 19, wherein the measure of antigen-antibody binding kinetics is a Kon rate; a Koff rate, or a combination thereof."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the APC is from one of the following: a human, a rabbit, a rat, a mouse, a sheep, an ape, a monkey, a goat; a dog, a cat, a camel, or a pig."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the removable capture substrate is a carboxylic acid (COOH) functionalized bead."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the removable capture substrate is capable of binding the protein secreted by the cell and the nucleic acids encoding the protein produced by the cell."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 or 14, further comprising washing the cell prior to exposing the protein secreted by the cell to a capture substrate."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the APC is a primary B cell or a memory B cell."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["A cell assay method, the method comprising:\n
a. distributing an antibody producing cell (APC) to a chamber having a solid wall defining the chamber and an aperture, wherein the volume of the chamber is from 100 pL to 100 nL and wherein the APC is in a first fluid;\n
b. replacing the first fluid with a second fluid while maintaining the APC in the chamber;\n
c. placing the antibodies produced by the APC in fluid communication with an antigen; and\n
d. determining the antigen-antibody binding kinetics of the antibodies produced by the APC with the antigen, wherein the measure of binding kinetics is one or more of: a Kon rate, a Koff rate, and a KD."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 26, wherein the measure of antigen-antibody binding kinetics is one or both of: a Kon rate; and a Koff rate."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 26, further comprising the step of adding a removable capture substrate to the chamber to capture the antibodies produced by the APC prior to placing the antibodies produced by the APC in fluid communication with an antigen."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 26 or 28, wherein placing the antibodies produced by the APC in fluid communication with an antigen comprises flowing a fluorescently labeled antigen through the chamber."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 or 26, further comprising collecting the mRNA from the cell for a reverse transcription polymerase chain reaction (RT-PCR) reaction to amplify the heavy and light chain genes."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 or 26, further comprising adding the cell to a reverse transcription polymerase chain reaction (RT-PCR) reaction to amplify the heavy and light chain genes."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4 or 26, wherein the determining the antigen-antibody binding kinetics comprises fluorescence imaging of antigen-antibody binding."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["A method of assaying for a binding interaction between a protein secreted by a cell and a biomolecule, the method comprising:\n
(a) retaining the cell secreting the protein within a chamber having a solid wall defining the chamber, and an aperture, wherein the volume of the chamber is from 100 pL to 100 nL;\n
(b) exposing the protein secreted by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein secreted by the cell and wherein the capture substrate is operable to bind the protein secreted by the cell to produce a bound protein;\n
(c) flowing over the bound protein a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate and the bound protein; and\n
(d) determining a binding kinetic measurement between the protein secreted by the cell and the biomolecule within the chamber based on an interaction between the biomolecule and the bound protein in (c), wherein the binding kinetic measurement is a Kon; a Koff; a KD, or a combination thereof."],"number":33,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the cell is a single cell."],"number":34,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the cell is an antibody producing cell (APC), the protein secreted by the cell is an antibody and the biomolecule is multiple different antigens."],"number":35,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 33, wherein the cell is maintained in the chamber with the capture substrate."],"number":36,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein steps (a)-(c) are carried out on the same device, in a plurality of chambers, each having a solid wall defining the chamber, an inlet and an outlet, and a plurality."],"number":37,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 33, wherein steps (a)-(c) are carried out on the same device, in a plurality of chambers, each having a solid wall defining the chamber, an inlet and an outlet."],"number":38,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}