{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"054-304-984-922-413","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"054-304-984-922-413","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8756,"type":"PATENT","title":"Princeton University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5503,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8201,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners = \"Princeton University\", \" University Princeton\", \"Princeton Univ\", \"Univ Princeton\", \" Princet* Univ*\", \"Trustees Univ* Princet*\".
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Total patents= 4986
Search applicants and owners = \"Princeton University\", \" University Princeton\", \"Princeton Univ\", \"Univ Princeton\", \" Princet* Univ*\", \"Trustees Univ* Princet*\".
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Total patents= 4986
a 2-photon or confocal laser scanning microscope (LSM) configured to scan the sample, comprising:\n\na plurality of scanning mirrors; and\na plurality of detectors;\n
a particle-holding device with the particle coupled to the particle-holding device, the particle-holding device being coupled to a stage with X-Y and Z position control;\n
a tracking module comprising:\n\na tracking excitation laser,\nX-Y radiation-gathering components configured to detect deviations of the particle in an X-Y direction,\nZ radiation-gathering components configured to detect deviations of the particle in a Z direction, and\na processor, coupled to the X-Y and Z radiation gathering components, configured to generate control signals to drive the stage X-Y and Z position controls to track movement of the particle; and\n
a synchronization module configured to synchronize the LSM and tracking module by:\n"],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 1 further comprising a processing module configured to generate a 3D image showing the 3D trajectory of a particle using the LSM pixel stamped with the stage position."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 2 further comprising a pixel to voxel conversion module configured to convert each detector readout from a lab frame to a three dimensional voxel in a sample frame based on known magnification of the LSM system and a piezoelectric stage position at each time point during the scan of the sample."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 3 further comprising an interpolation module configured to generate interpolated data including average oversampled voxels and interpolated unsampled voxels."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 4 further comprising a smoothing module configured to smooth the interpolated data."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 4 further comprising an image generating module configured to generate a 3D image showing movement of the particle based on the interpolated data."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 1 wherein the tracking excitation laser is mounted parallel to an optical table plane and the X-Y radiation gathering components are mounted vertically with respect to the optical table plane."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 7 wherein each X-Y radiation gathering component has a mirror configured to reflect light from the plane of the optical table to a vertical orientation."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The system of claim 7 further comprising a vertical mounting surface having X radiation gathering components mounted to one side and Y radiation gathering components mounted to an opposite side."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A method for non-invasively tracking a particle in a sample, comprising:\nreceiving current scanning mirror positions and detector readouts from the LSM;\nreceiving a current stage position from the tracking module; and\ngenerating an LSM pixel stamped with a stage position.\n
providing a 2-photon or confocal laser scanning microscope (LSM) with a plurality of scanning mirrors and a plurality of detectors configured to scan the sample;\n
providing a particle-holding device with the particle coupled to the particle-holding device, the particle-holding device being coupled to a stage with X-Y and Z position control;\n
providing a tracking module configured to:\n"],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 10 further comprising generating a 3D image showing the 3D trajectory of a particle using the LSM pixel stamped with the stage position."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 11 further comprising converting each detector readout from a lab frame to a three dimensional voxel in a sample frame based on known magnification of the LSM system and a piezoelectric stage position at each time point during the scan of the sample."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12 further comprising generating interpolated data including average oversampled voxels and interpolated unsampled voxels."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 further comprising smoothing the interpolated data."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 further comprising generating a 3D image showing movement of the particle based on the interpolated data."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 10 wherein the tracking excitation laser beam is oriented parallel to an optical table plane and X-Y radiation gathering components are mounted vertically with respect to the optical table plane."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16 wherein each X-Y radiation gathering component has a mirror configured to reflect light from the plane of the optical table to a vertical orientation."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16 further comprising providing a vertical mounting surface having X radiation gathering components mounted to one side and Y radiation gathering components mounted to an opposite side."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["A system for non-invasively tracking a particle in a sample, comprising:\ngenerate a tracking excitation laser beam;\ndetect deviations of the particle in an X-Y and Z direction; and\ngenerate control signals to drive the stage X-Y and Z position controls to track movement of the particle; and synchronizing the LSM and tracking module by:\nreceiving current scanning mirror positions and detector readouts from the LSM;\nreceiving a current stage position from the tracking module; and\ngenerating an LSM pixel stamped with a stage position.\n
an imaging module configured to scan the sample comprising:\n\na laser scanning microscope (LSM);\na plurality of scanning mirrors; and\na plurality of single point detectors;\n
a microscope stand comprising:\n\na sample holder containing the sample; and\na stage configured to provide X-Y and Z position control;\n
a tracking module comprising:\n\na tracking excitation laser;\nX-Y radiation-gathering components configured to detect deviations of the particle in an X-Y direction;\nZ radiation-gathering components configured to detect deviations of the particle in a Z direction; and\na processor configured to generate control signals to drive the stage X-Y and Z position controls to track movement of the particle; and\n
a synchronization module configured to synchronize the imaging module and tracking module by:\n"],"number":19,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}receiving current scanning mirror positions and detector readouts from the imaging module;\nreceiving a current stage position from the tracking module; and\ngenerating an LSM pixel stamped with a stage position.\n