{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"053-355-544-224-99X","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"053-355-544-224-99X","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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a VioB gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:3 and having 2,3-diaminopropionate synthase activity,\n
a VioC qene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:4 and havinq L-arqinine hydroxylase activity,\n
a VioD gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:5 and having capreomycidine synthase activity,\n
a VioE gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:6 and having permease activity,\n
a VioF gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:7 and having non-ribosomal peptide synthetase carrier protein activity conveying L-2,3-diaminopropionate to the VioA gene product,\n
a VioG gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:8 and having non-ribosomal peptide synthetase pentapeptide cyclization activity,\n
a VioH gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:9 and having Tyoe II thioestererase activity,\n
a Viol gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:10 and having non-ribosomal peptide synthetase carrier protein activity conveyinq a desaturated 2,3-diaminopropionate-comprising tetrapeptide to the VioG gene product,\n
a VioJ gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:11 and having 2,3-diaminopropionyl α,β-desaturase activity,\n
a VioK gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:12 and having ornithine cyclodeaminase activity,\n
a VioL gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:13 and having carbamoyltransferase activity,\n
a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity,\n
a VioN gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:15 and having β-lysine addition activity,\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity,\n
a VioP gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:17 and having lysine 2,3-aminomutase activity,\n
a VioQ gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:19 and having capreomycidine hydroxylase activity,\n
a VioR gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:20 and having transcriptional regulatory activity,\n
a VioS gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:21 and having viomycin-phosphate phosphatase activity, and\n
a VioT gene product having the amino acid sequence set forth in SEQ ID NO:22 and having transcriptional regulatory activity."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 which further encodes\n
a VioB gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:3 and having 2,3-diaminopropionate synthase activity,\n
a VioC gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:4 and having L-arginine hydroxylase activity,\n
a VioD gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:5 and having capreomycidine synthase activity, and\n
a VioG gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:8 and having non-ribosomal peptide synthetase pentapeptide cyclization activity, and additionally encodes at least one other gene product selected from the group of gene products consisting of\n
a VioE gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:6 and having permease activity,\n
a VioF gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:7 and having non-ribosomal peptide synthetase carrier protein activity conveying L-2,3-diaminopropionate to the VioA gene product,\n
a VioH gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:9 and having Type II thioestererase activity,\n
a Viol gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:10 and having non-ribosomal peptide synthetase carrier protein activity conveying a desaturated 2,3-diaminopropionate-comprising tetrapeptide to the VioG gene product,\n
a VioJ gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:11 and having 2,3-diaminopropionyl α,β-desaturase activity,\n
a VioK gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:12 and having ornithine cyclodeaminase activity,\n
a VioL gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:13 and having carbamoyltransferase activity,\n
a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity,\n
a VioN gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:15 and having β-lysine addition activity,\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity,\n
a VioP gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:17 and having lysine 2,3-aminomutase activity,\n
a VioQ gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:19 and having capreomycidine hydroxylase activity,\n
a VioR gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:20 and having transcriptional regulatory activity,\n
a VioS gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:21 and having viomycin-phosphate phosphatase activity, and\n
a VioT gene product having the amino acid sequence set forth in SEQ ID NO:22 and having transcriptional regulatory activity."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 which further encodes a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity,\n
a VioN gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:15 and having β-lysine addition activity,\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity, and\n
a VioP gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:17 and having lysine 2,3-aminomutase activity."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 which further encodes a VioC gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:4 and having L-arginine hydroxylase activity and a VioD gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:5 and having capreomycidine synthase activity."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The isolated and purified nucleic acid molecule of claim 1 further comprising genes that encode for a gene product selected from the group consisting of a VioF gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:7 and having non-ribosomal peptide synthetase carrier protein activity conveying L-2,3-diaminopropionate to the VioA gene product,\n
a VioG gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:8 and having non-ribosomal peptide synthetase pentapeptide cyclization activity,\n
a Viol gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:10 and having non-ribosomal peptide synthetase carrier protein activity conveying a desaturated 2,3-diaminopropionate-comprising tetrapeptide to the VioG gene product,\n
a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity, and\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 comprising a nucleic acid sequence which further encodes one or more polypeptides having an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and SEQ ID NO:22."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 wherein the nucleic acid sequence has at least 80% nucleic acid sequence identity with the nucleic acid sequence set forth in SEQ ID NO:1 from position 415 through position 6786."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 9, wherein the nucleic acid sequence identity is at least 90% with the nucleic acid sequence set forth in SEQ ID NO:1 from position 415 through position 6786."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 9, wherein the nucleic acid sequence identity is at least 95% with the nucleic acid sequence set forth in SEQ ID NO:1 from position 415 through position 6786."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 which is from a wild-type Streptomyces sp. ATCC11861, Streptomyces californicus, or Streptomyces olivoreticulis subsp. Olivoreticuli. "],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 12 which is from wild-type Streptomyces sp. ATCC11861."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid molecule of claim 1 comprising a viomycin gene cluster."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["An expression cassette comprising the nucleic acid molecule of claim 1 operably linked to a promoter functional in a host cell, wherein the expression of the cassette in a host transformed with the cassette permits the production of viomycin by the host cell."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 15, wherein the nucleic acid molecule further encodes at least one additional gene product selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22, and wherein the expression of the cassette in a host cell comprising the nucleic acid permits the production of viomycin by the host cell."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 16, wherein the nucleic acid molecule further encodes the gene products comprising SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22, and wherein the expression of the cassette in a host cell comprising the nucleic acid permits the production of viomycin by the host cell."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["An isolated, recombinant, host cell transformed with at least the nucleic acid molecule of claim 1 and capable of expressing the encoded VioA gene product."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18 in which viomycin levels are increased relative to the levels in a corresponding nonrecombinant host cell."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18 wherein the recombinant host cell is a prokaryote or a eukaryote host cell."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18 wherein the recombinant host cell is a cell of the genus Streptomyces. "],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18 wherein the recombinant host cell is Streptomyces sp. ATCC11861."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim of claim 18 wherein the nucleic acid molecule further encodes a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity,\n
a VioN gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:15 and having β-lysine addition activity,\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity, and\n
a VioP gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:17 and having lysine 2,3-aminomutase activity."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 23 which is a Saccharothrix mutabolis subsp. capreolus host cell."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18 wherein the nucleic acid molecule further encodes a VioC gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:4 and having L-arginine hydroxylase activity and a VioD gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:5 and having capreomycidine synthase activity."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 25 wherein the recombinant host cell is E. coil. "],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 18, wherein the gene cluster further encodes and expresses at least one gene product selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22, and wherein the expression of the nucleic acid in a host cell comprising the nucleic acid permits the production of viomycin by the host cell."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant host cell of claim 27, wherein the gene cluster further encodes and expresses the gene products comprising SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22, and wherein the expression of the nucleic acid in a host cell comprising the nucleic acid permits the production of viomycin by the host cell."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["A method for preparing viomycin or a pharmaceutically acceptable salt thereof comprising transforming a host cell with the expression cassette of claim 15, culturing the host cell in a culture medium containing assimilable sources of carbon, nitrogen and inorganic salts under aerobic fermentation conditions to yield an increase in the viomycin relative to the level of the viomycin produced by a corresponding untransformed host cell."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein the host cell is a prokaryote or a eukaryote host cell."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein the host cell is a cell of the genus Streptomyces. "],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein the host cell is Streptomyces sp. ATCC11861."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein the transforming expression cassette encodes a VioM gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:14 and having non-ribosomal peptide synthetase condensation β-lysine transferase activity,\n
a VioN qene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:15 and havinq β-lysine addition activity,\n
a VioO gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:16 and having non-ribosomal peptide synthetase carrier protein β-lysine thioesterification activity, and\n
a VioP gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:17 and having lysine 2,3-aminomutase activity."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 33 wherein the host cell is a prokaryote or a eukaryote host cell."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 33 wherein the host cell is a cell of the genus Streptomyces. "],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 33 wherein the host cell is Streptomyces sp. ATCC11861."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 33 wherein the host cell is Saccharothrix mutabolis subsp. capreolus. "],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein expression cassette encodes a VioC gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:4 and having L-arqinine hydroxylase activity and a VioD gene product having an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:5 and having capreomycidine synthase activity."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38 wherein the host cell is a prokaryote or a eukaryote host cell."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38 wherein the host cell is a cell of the genus Streptomyces. "],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38 wherein the host cell is Streptomyces sp. ATCC11861."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38 wherein the host cell is E. coil. "],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 29 wherein the expression cassette encodes the viomycin gene cluster."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 43 wherein the host cell is a prokaryote or a eukaryote host cell."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 43 wherein the host cell is a cell of the genus Streptomyces. "],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 43 wherein the host cell is Streptomyces sp. ATCC11861."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["An isolated and purified polypeptide having the amino acid sequence of the VioA gene product set forth in SEQ ID NO:2."],"number":47,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}