{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"045-935-447-413-626","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"045-935-447-413-626"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12063,"type":"PATENT","title":"Wayne State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2773,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8588,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Wayne State Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2610
Search Applicants and Owners separately:Wayne State Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2610
\n\nZ1-[L]m-[T1]-[L]n-[T2]-[L]p-[T3]-[L]q-[T4]-[L]r-[T5]-[L]s-Z2 V (5′→3′)\n\nwherein, independently for each occurrence,\n
T1, T2, T3, T4 and T5 are selected from the group consisting of SEQ ID NOs: 4, 8, 9, 10, 11, 43, 44, 45, 46, 47, 48, 49, 52, 53, 54, 55, 56, 57, 58 and 59;\n
L is a nucleic acid selected from the group consisting of adenosine, cytidine, guanosine and uridine; optionally substituted with one substituent selected from the group consisting of biotin, fluorescein, tetrachlorofluorescein, hexachlorofluorescein, Cy3 amidite, Cy5 amidite, digoxigenin, TEXAS RED® maleimide and tetramethylrhodamine;\n
Z1 and Z2 are selected from the group consisting of biotin, fluorescein, tetrachlorofluorescein, hexachlorofluorescein, Cy3 amidite, Cy5 amidite, digoxigenin and resin beads; and\n
m, n, p, q, r and s are independently an integer from 0 to 40."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein T1 is SEQ ID NO 4; T2 is SEQ ID NO 8; T3 is SEQ ID NO 9; T4 is SEQ ID NO 10; and T5 is SEQ ID NO 11."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 2, wherein p is 2, q is 17, r is 15, Z1 is fluorescein, Z2 is biotin, and each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein T1 is SEQ ID NO 43; T2 is SEQ ID NO 44; T3 is SEQ ID NO 55; T4 is SEQ ID NO 56; and T5 is SEQ ID NO 57."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 4, wherein n is 2, r is 3, Z1 is fluorescein, Z2 is biotin, or each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 4, wherein n is 2, r is 3, Z1 is fluorescein, Z2 is biotin, and each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein T1 is SEQ ID NO 45; T2 is SEQ ID NO 46; T3 is SEQ ID NO 54; T4 is SEQ ID NO 58; and T5 is SEQ ID NO 59."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 7, wherein n is 16, r is 6, Z1 is fluorescein, Z2 is biotin, or each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 7, wherein n is 16, r is 6, Z1 is fluorescein, Z2 is biotin, and each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein T1 is SEQ ID NO 47; T2 is SEQ ID NO 48; T3 is SEQ ID NO 49; T4 is SEQ ID NO 52; and T5 is SEQ ID NO 53."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 10, wherein n is 7, p is 37, r is 7, Z1 is fluorescein, Z2 is biotin, or each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 10, wherein n is 7, p is 37, r is 7, Z1 is fluorescein, Z2 is biotin, and each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein Z1 is fluorescein."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein Z2 is biotin."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein each L is, independently for each occurrence, selected from the group consisting of unsubstituted adenosine, unsubstituted cytidine, unsubstituted guanosine and unsubstituted uridine."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein m and s are integers between 0-25."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 1, wherein m and s are integers between 5-15."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["A method of identifying an inhibitor of protein synthesis comprising\n
measuring the fluorescence of the nucleic acid of claim 1, thereby establishing a first fluorescence reading;\n
contacting a test compound with said nucleic acid, and measuring the resulting fluorescence, thereby establishing a second fluorescence reading;\n
determining the difference between said first fluorescence reading and said second fluorescence reading;\n
selecting the compound wherein the difference between said first fluorescence reading and said second fluorescence reading is non-zero, thereby identifying said agent;\n
assaying the inhibitory properties of the agent by administering it to a cell, a cell extract or purified ribosomes; and\n
detecting protein synthesis; wherein a decrease in protein synthesis indicates that the agent is an inhibitor of protein synthesis."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein assaying the inhibitory properties of the agent comprises detecting protein synthesis."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein assaying the inhibitory properties of the agent comprises determining the inhibitor constant for inhibiting mRNA translation."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of identifying an agent which binds to the nucleic acid of claim 1, comprising the steps of:\n
measuring the fluorescence of said nucleic acid, thereby establishing a first fluorescence reading;\n
contacting a test compound with said nucleic acid, and measuring the resulting fluorescence, thereby establishing a second fluorescence reading;\n
determining the difference between said first fluorescence reading and said second fluorescence reading; and\n
selecting the compound wherein the difference between said first fluorescence reading and said second fluorescence reading is non-zero, thereby identifying said agent."],"number":21,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}