{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"033-695-300-626-162","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"033-695-300-626-162"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11649,"type":"PATENT","title":"Tufts University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3596,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8315,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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a fiber optic array comprising a plurality of individual fiber strands, each strand having a distal end and a proximal end and a core, said core diameter sized to accommodate a predetermined cell type, said core capable of being anisotropically etched;
a microwell formed on an end surface of the distal end of each of said strands, said microwell formed by anisotropic etching said core of each of said strands, said microwell sized to accommodate insertion of a single cell of said cell type;wherein each of said single cells in said microwells is optically coupled and in optical communication with one of said fiber strands."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein said microwells have an internal diameter ranging from about 1 um to about 200 um, a depth ranging from about 0.25 um to about 200 um, and a volume ranging between about 1 fL to about 5 nanoliter."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein the diameter of said core of said fiber strands ranges from about 1 um to about 200 um."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein an interior surface of said microwell is coated with a thin film of a biologically compatible material selected from a group consisting of collagen, fibronectin, polylysine, polyethylene glycol, polystyrene, and metal."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 4 wherein said metal comprises gold, platinum or palladium."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein an indicator compound is applied to an interior surface of said microwell for detecting cell responses to chemical or biological stimulation."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein at least one cell type is inserted in at least one microwell, said cell type selected from a group consisting of invertebrate neurons, mammalian neurons, adrenomedullary cells, lymphocytes, erythrocytes, E.coli bacteria, staphylococcus bacteria, myoblasts, neutrophils, erthroblasts, osteoblasts, chodrocytes, basophils, eosinophils, melanocytes, epithelial cells, epithedial cells, adipocytes, and naturally occuring or genetically engineered cells."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 7 wherein an indicator compound is attached or inserted into said cell."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 1 wherein a plurality of cells are inserted in a plurality of microwells."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 9 wherein said plurality of cells is comprised of at least two cell populations which are randomly mixed and inserted in said microwells."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor of claim 10 wherein each cell population is encoded with at least one optically interrogatable material selected from a group consisting of a fluorophore, a chromophore, and a chemiluminescent or bioluminescent compound or material."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["An apparatus for detecting the response of individual cells to at least one analyte of interest comprising:
a) a biosensor array comprising
i) a fiber optic array comprising a plurality of individual fiber strands, each strand having a distal end and a proximal end and a core, said core diameter sized to accommodate a predetermined cell type, said core capable of being anisotropically etched,
ii) a microwell formed on an end surface of the distal end of each of said strands, said microwell formed by anisotropic etching said core of each of said strands, said microwell sized to accommodate insertion of a single cell of said cell type,
iii) a plurality of cells inserted in said microwells wherein each of said inserted cells in said microwells is optically coupled and in optical communication with one of fiber strands, and
iv) a detector optically coupled to a proximal end of said strands, said detector capable of detecting an optical response of said cells inserted in said microwells, said response being characteristic of the presence or absence of said analyte."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 12 further comprising an excitation light energy component coupled to said proximal end of said strands, said light energy component for illuminating said cells in said microwells."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 12 further comprising an image capturing system for capturing images of a plurality of detected optical responses from each individual cell, said images being characteristic of the presence or absence of said analyses."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 12 wherein said image capturing system comprises a CCD camera, a computer interface and a programmable computer."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}