{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"033-662-853-045-877","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"033-662-853-045-877","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8635,"type":"PATENT","title":"MIT Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":30193,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8198,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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wherein the nucleic acid construct does not comprise Oct 3/4, Sox 2 and Klf4 ligated across the 2A sequence of foot-and-mouth disease virus in the order of Oct 3/4, Klf4 and Sox2; and wherein the nucleic acid construct does not comprise c-Myc."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 1, further comprising a fourth coding region that encodes a fourth reprogramming factor."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 1, wherein:\n
the first or second self-cleaving peptide is a viral 2A peptide or the first and second self-cleaving peptide are a viral 2A peptide."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["An expression cassette comprising: the nucleic acid construct of claim 1 operably linked to a promoter, wherein the promoter drives transcription of a polycistronic message that encodes the reprogramming factors."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["An expression vector comprising: the expression cassette of claim 4."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["An isolated mammalian cell comprising: the expression cassette of claim 4."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 3, wherein the viral 2A peptide is an aphthovirus 2A peptide."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 3, wherein the viral 2A peptide is selected from the group consisting of: a foot-and-mouth disease virus (FMDV) 2A peptide, an equine rhinitis A virus (ERAV) 2A peptide, a Thosea asigna virus (TaV) 2A peptide, a porcine teschovirus-1 (PTV-1) 2A peptide, a Theilovirus 2A peptide, and an encephalomyocarditis virus 2A peptide."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 1, wherein the coding regions encode a set of reprogramming factors selected from the group consisting of: (i) Oct4, Klf4, and Sox2; (ii) Oct4, Nanog, and Lin28; (iii) Oct4, Nanog, and Sox2."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 1, wherein the coding regions encode a set of reprogramming factors selected from the group consisting of: Oct4, Klf4, and Sox2."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid construct of claim 1, wherein:\n
(a) the reprogramming factors are selected from the group consisting of: Oct4, Nanog, Sox2, Klf4, and Lin28, and c Myc; or\n
(b) the construct does not encode one of the reprogramming factors selected from the group consisting of: Oct4, Klf4, Sox2, Lin28, and Nanog."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The isolated mammalian cell of claim 6, wherein the cell expresses three reprogramming factors from the expression cassette."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The isolated mammalian cell of claim 6, wherein the cell expresses three reprogramming factors from the expression cassette, wherein the three reprogramming factors are not sufficient to reprogram mammalian fibroblasts with an efficiency of at least 0.05%."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The isolated mammalian cell of claim 6, wherein:\n
(a) the cell is selected from the group consisting of: a somatic cell, a terminally differentiated somatic cell, and an iPS cell; or\n
(b) the cell is a human cell; or\n
(c) the cell further comprises a reporter gene integrated at a locus whose activation serves as a marker of reprogramming to pluripotency."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The isolated mammalian cell of claim 6, wherein the cell expresses three reprogramming factors from the expression cassette, wherein the three reprogramming factors are sufficient to reprogram mammalian fibroblasts with an efficiency of at least 0.05%."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 4, further comprising one or more sites that mediate integration into the genome of a mammalian cell."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The expression vector of claim 5, wherein\n
(a) the vector is retroviral; or\n
(b) the promoter is inducible."],"number":17,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}