{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"025-716-956-228-060","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"025-716-956-228-060"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10838,"type":"PATENT","title":"Michigan State University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5685,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8287,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
Search Applicants and Owners separately: Michigan AND State AND Univ*
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4400
a) providing;\n\ni) a plant biomass comprising a hemicellulose material; and\nii) an enzyme mixture comprising at least about 5% by weight of isolated Aspni5|43342 α-xylosidase, or an α-xylosidase with at least 95% sequence identity to SEQ ID NO:1, and at least 50% by weight of a cellulase mixture comprising cellobiohydrolase, endoglucanase, β-1,4-glucanase, β-glucosidase, endoxylanase, and β-xylosidase; and\n
b) incubating said biomass with said enzyme mixture using at least about 2 mg of an isolated α-xylosidase per gram biomass for a time and under conditions sufficient to create a depolymerized hemicellulose material;\n"],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the α-xylosidase with at least 95% sequence identity to SEQ ID NO:1 has one or more conservative amino acid substitutions."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said method further comprises pretreating said plant biomass with alkaline hydrogen peroxide, acid, ammonia, ionic liquids, steam or a combination thereof."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said depolymerized hemicellulose material is at least 50% depolymerized into the plurality of free fermentable xylose and glucose residues."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said cellulase mixture further comprises at least one enzyme selected from the group of α-fucosidase, β-galactosidase, α-arabinosidase, α-glucuronidase polysaccharide mono-oxygenase, esterase and combinations thereof."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said isolated α-xylosidase lacks a quaternary structure."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said isolated α-xylosidase is a secreted α-xylosidase."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said isolated α-xylosidase has a pH optimum of approximately 4.0."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said isolated α-xylosidase comprises or is derived from a fungal extracellular extract."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein said fungal extracellular extract is an Aspergillus niger extracellular extract."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said plant biomass comprises a dicot xyloglucan."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said plant biomass comprises a monocot xyloglucan."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said plant biomass comprises grass xyloglucan or corn stover."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said incubating is performed at a temperature ranging from approximately 40° C. to 50° C."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said incubating is performed at a pH of approximately 4-5."],"number":15,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}wherein said depolymerized hemicellulose material comprises a plurality of free fermentable xylose and glucose residues.\n