{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"023-461-923-298-439","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"023-461-923-298-439"},"entityMetadata":{"linkedIds":{"empty":false},"tags":[],"collections":[{"id":8590,"type":"PATENT","title":"Harvard University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":21990,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[],"sharedType":"PUBLISHED","hasLinkedSavedQueries":false,"savedQueries":[],"created":"2015-11-04T04:19:00Z","updated":"2022-09-08T02:14:43Z","lastEventDate":"2022-09-08T02:14:43Z"},{"id":8838,"type":"PATENT","title":"Univ of Illinois Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10245,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8209,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
a) transformimg a host cell which expresses mutant Himar1 transposase coding sequence with a vector comprising a promoter active in said host cell operably linked to a coding region for said transposase polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:4; SEQ ID NO:6; SEQ ID NO:8; SEQ ID NO:10; SEQ ID NO:12; SEQ ID NO:14, to produce a recombinant host cell; and
b) culturing the recombinant host cell under conditions wherein said mutant transposase protein is expressed."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11 wherein said host cell is selected from the group consisting of bacterial cells, yeast cells, insect cells, and mammalian cells."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12 wherein said host cell is E. Coli. "],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["An expression vector comprising a nucleic acid encoding a mutant Himar1 transposase protein consisting of the amino acid sequence as set forth in SEQ ID NO:6."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["An expression vector comprising a nucleic acid encoding the mutant Himar1 transposase protein consisting of the amino acid sequence as set forth in SEQ ID NO:10."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A recombinant host cell transformed to contain the expression vector of claim 14 ."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["A recombinant host cell transformed to contain the expression vector of claim 15 ."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["A method for in vitro transposition, the method comprising the steps of:
combining a donor DNA molecule comprising a transposable DNA sequence of interest with a target DNA molecule and a mutant Himar1 transposase o f claim 1 under conditions such that the mutant Himar1 transposase mediates transposition of the DNA sequence of interest to the target DNA."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18 wherein the mutant transposase consists of the amino acid sequence as given in SEQ ID NO:6."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18 wherein the mutant transposase consists of the amino acid sequence as given in SEQ ID NO:10."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18 wherein said DNA sequence of interest comprises a primer sequence for DNA sequencing."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A method for in vivo transposition, the method comprising the steps of:
introducing a donor DNA molecule comprising a transposable DNA sequence of interest and a modified Himar1 transposon comprising a mutant Himar1 transposase of claim 1 into an organism, cell or tissue under conditions such that the mutant transposase mediates transposition of the DNA sequence of interest to the chromosome of the organism or cell or tissue."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22 , wherein the mutant Himar1 transposase consist of the amino acid sequence as given in SEQ ID NO:6."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 22 , wherein the mutant Himar1 transposase consists of the amino acid sequence as given SEQ ID NO:10."],"number":24,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}