{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"021-891-550-805-828","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"021-891-550-805-828","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8635,"type":"PATENT","title":"MIT Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":30193,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8198,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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Note: over 20k
an optically transparent material;\n
a substrate coupled to the optically transparent material; and\n
a scaffold having dimensions of at least 10 μm in three-dimensional space;\nwherein\n
the substrate comprises a plurality of posts;\n
the scaffold contacts the substrate, the posts, and the optically transparent material;\n
the scaffold comprises a solid or semi-solid biological or biocompatible polymer;\n
the substrate comprises a first fluid-flow path and a second fluid-flow path;\n
the first fluid-flow path does not intersect with the second fluid-flow path;\n
the scaffold is disposed between the first fluid-flow path and the second fluid-flow path;\n
the posts are arranged in a staggered array; and\n
the staggered array of posts is disposed between the first fluid flow path and the second fluid flow path."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the first fluid-flow path and the second fluid-flow path are channels in the substrate."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the substrate comprises plastic."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the substrate comprises PDMS."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the substrate is modified."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 5, wherein the modified substrate is a result of exposure of the substrate to poly-D-lysine, or exposure of the substrate to plasma."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the scaffold comprises a first biological entity."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, further comprising a pressure regulator."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the first fluid-flow path or the second fluid-flow path comprises a resistance channel."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the first fluid-flow path has different dimensions than the second fluid flow path."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 1, wherein the scaffold comprises a solid or semi-solid biocompatible polymer; and the solid or semi-solid biocompatible polymer allows the passage of a second biological entity."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 11, wherein the scaffold comprises collagen, agarose, gelatin, fibronectin, fibrin, laminin, or a peptide; and the second biological entity comprises a eukaryotic cell."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The device of claim 11, wherein the scaffold comprises collagen; and the second biological entity is selected from the group consisting of a growth factor, a cytokine, a hormone, an antibody, and an enzyme."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method of measuring directed migration of a cell, comprising the steps of:\n
a) providing a device of claim 1;\n
b) introducing a cell into the first fluid-flow path;\n
c) introducing a biological entity into the second fluid-flow path; and\n
d) measuring the directed migration of the cell into the scaffold."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the biological entity is selected from the group consisting of prokaryotic cells, eukaryotic cells, growth factors, cytokines, hormones, antibodies, drugs, and enzymes."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the cell is a neutrophil, and the biological entity is an endothelial cell."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the cell is an endothelial cell; the biological entity is a second cell; the second cell is a tumor cell."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the cell and the second cell are obtained from the same subject."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["A method of measuring blood vessel formation, comprising the steps of:\n
a) providing a device of claim 1;\n
b) introducing an endothelial cell or endothelial cell precursor into the first fluid-flow path;\n
c) introducing a biological entity into the second fluid-flow path; and\n
d) measuring the formation of a blood vessel in the scaffold."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 19, wherein the biological entity is selected from the group consisting of prokaryotic cells, eukaryotic cells, growth factors, cytokines, hormones, antibodies, drugs, and enzymes."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["A method of measuring permeability, comprising the steps of\n
a) providing a device of claim 1;\n
b) introducing a first substance into the first fluid-flow path; and\n
c) measuring the permeability of the first substance into the scaffold."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the first substance is a fluid or a small molecule."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the scaffold comprises a cell monolayer."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the first substance comprises a cell."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein the cell is an endothelial cell."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the scaffold comprises a second cell."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 26, wherein the second cell is a tumor cell."],"number":27,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}