{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"020-407-766-554-69X","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"020-407-766-554-69X","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
Select more for logical variants
Add to collection
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
Select more for logical variants
Add to collection
(a) obtaining a synchronous population of cells that are cultured from embryonic stem cells, and which are characterized by an early rosette morphology and are Sox1−, Pax6+;\n
(b) culturing the cells obtained in (a) in a neural basal medium with fibroblast growth factor-8 (FGF8), wherein resulting cells are Pax6+, Sox1+ and engrailed 1 positive (EN-1+) neuroepithelial cells;\n
(c) expanding the cells from step (b) in a neural basal medium with sonic hedgehog (SHH) and FGF8, wherein resulting cells are dopaminergic neural precursors; and\n
(d) culturing the cells from step (c) in a neural basal differentiation medium without SHH and FGF8 until resulting cells express tyrosine hydroxylase (TH), aromatic acid decarboxylase (AADC), EN-1, vesicular monoamine transporter 2 (VMAT2) and dopamine transporter (DAT) but do not express dopamine beta hydroxylase (DbH) and phenylethanolamine N-methyltransferase (PNMT), and produce dopamine."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the exposure to SHH is between 6-7 days."],"number":2,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}