{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"014-151-969-371-793","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"014-151-969-371-793","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11855,"type":"PATENT","title":"University of Montpellier Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":11808,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8467,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
a) providing a Deinococcus bacterium;\n
b) introducing into said bacterium, a recombinant nucleic acid molecule encoding PDC from Zymomonas and ADH from Zymomonas; wherein the PDC and ADH coding sequences are placed in an operon in the recombinant nucleic acid molecule, and wherein said recombinant nucleic acid molecule is integrated into the genome of the bacterium; and\n
c) optionally further treating the bacterium to reduce activity of the LDH gene and selecting a bacterium having an at least partially inactivated LDH gene."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["A plasmid construct selected from the group consisting of pI3-DR-P-PDC-ADH, pI3-P-PDC-P-ADH and pI3-DR-P-PDCtag-P-ADHtag."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A recombinant Deinococcus bacterium, wherein said bacterium contains a recombinant nucleic acid construct encoding a pyruvate decarboxylase (PDC) from Zymomonas and an alcohol dehydrogenase (ADH) from Zymomonas, wherein the PDC and ADH coding sequences are placed in an operon in the recombinant nucleic acid construct, and wherein said recombinant nucleic acid construct is integrated into the genome of the bacterium in place of an endogenous LDH gene."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing ethanol comprising cultivating a bacterium of claim 14 in the presence of an appropriate substrate, and collecting ethanol."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant Deinococcus bacterium according to claim 1, wherein said recombinant nucleic acid construct bacterium comprises pI3-DR-P-PDC-ADH."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The recombinant Deinococcus bacterium according to claim 1, wherein said recombinant nucleic acid construct comprises a pyruvate decarboxylase gene (PDC) from Zymomonas and an alcohol dehydrogenase (ADH) gene from Zymomonas in an operon, said PDC gene and said ADH gene being separated by a nucleic acid spacer sequence that contains a ribosomal binding sequence and said recombinant nucleic acid construct comprises a termination sequence after said PDC gene and said ADH gene."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 11, wherein said biofuel is ethanol and said substrate comprises plant biomass."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said substrate comprises plant biomass."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing ethanol comprising cultivating a bacterium of claim 1 in the presence of an appropriate substrate, and collecting ethanol."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein said substrate comprises plant biomass."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein said substrate is a culture medium comprising glucose."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said substrate is a culture medium comprising glucose."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The bacterium of claim 1, wherein recombinant nucleic acid construct comprises a tufA promoter operably linked to the PDC coding sequence."],"number":24,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}