{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"004-411-356-062-352","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"004-411-356-062-352","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10784,"type":"PATENT","title":"Purdue University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7369,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8254,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
conducting a protein-fragment complementation assay on the sample to form a protein-protein complex transiently under physiological conditions;\n
separating the complex from the sample using a moiety that specifically binds a fused form of the reporter protein but not either of the first and second proteins individually, and applying a washing condition to the sample that removes the first and second proteins in their free form while preserving the complex; and\n
quantitatively analyzing at least one protein of the complex using a mass spectrometry technique."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the reporter protein is a bimolecular fluorescence protein."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein separating comprises:\n
exposing the complexes to one or more solid supports, each solid support comprising the moiety that specifically binds the fused form of the reporter protein; and\n
washing away remaining components of the sample."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein the moiety is an antibody that specifically binds the fused form of the reporter protein."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, wherein the antibody is selected from the group consisting of an anti-fluorescence protein antibody, a nanobody and a variable single domain antibody fragment (VHH)."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein the fused form of the reporter protein comprises a first member of a binding pair, and the moiety is a second member of the binding pair."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein the solid supports are beads."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein prior to the analyzing step, the method further comprises:\n
eluting the complexes from the solid supports; and\n
digesting the proteins to from peptides."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein one protein of the complex is a kinase."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["A method for analyzing two transiently interacting proteins in a sample, wherein a first protein is fused with a first fragment of a reporter protein and a second protein is fused with a second fragment of said reporter protein, the method comprising:\n
conducting a protein-fragment complementation assay on the sample to form a protein-protein complex transiently under physiological conditions, and the assay is conducted in the presence of a competitor;\n
separating the complex from the sample using a moiety that specifically binds a fused form of the reporter protein but not either of the first and second proteins individually, and applying a washing condition to the sample that removes the first and second proteins in their free form while preserving the complex; and\n
quantitatively analyzing a protein of the complex using a mass spectrometry technique."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 10, wherein the reporter protein is a bimolecular fluorescence protein."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 10, wherein separating comprises:\n
exposing the complexes to one or more solid supports, each solid support comprising the moiety that specifically binds the fused form of the reporter protein; and\n
washing away remaining components of the sample."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein the moiety is an antibody that specifically binds the fused form of the reporter protein."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 13, wherein the antibody is selected from the group consisting of an anti-fluorescence protein antibody, a nanobody and a variable single domain antibody fragment (VHH)."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein the fused form of the reporter protein comprises a first member of a binding pair, and the moiety is a second member of the binding pair."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein the solid supports are beads."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein prior to the analyzing step, the method further comprises:\n
eluting the complexes from the solid supports; and\n
digesting the proteins to from peptides."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 10, wherein one protein of the complex is a kinase."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the analyzing step provides an amino acid modification status of at least one protein in the complex."],"number":19,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}